Chromatografické stanovenie B-komplexu

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Chromatografické stanovenie B-komplexu

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dc.contributor.advisor Kramářová, Daniela
dc.contributor.author Kožáková, Zuzana
dc.date.accessioned 2010-07-18T09:20:46Z
dc.date.available 2010-07-18T09:20:46Z
dc.date.issued 2009-05-19
dc.identifier Elektronický archiv Knihovny UTB cs
dc.identifier.uri http://hdl.handle.net/10563/10909
dc.description.abstract Cieľom tejto práce bolo otestovať možnosti stanovenia B-komplexu pomocou vysokoúčinnej kvapalinovej chromatografie a v prípade úspešného stanovenia navrhnúť metodiku na vybrané doplnky stravy. Vitamíny skupiny B boli stanovené metódou HPLC s UV detekciou pri 204 a 220 nm. Separácia prebehla na kolóne SUPELCOSIL LC 8(15 cm x 4,6 mm; 5 um). Mobilná fáza bola zložená z metanolu a KH2PO4 (pH 7) v pomere 10:90. Elúcia prebehla izokraticky, zvolený prietok bol 0,8 ml.min-1. Uvedená metodika bola aplikovaná na šesť vybraných doplnkov stravy. cs
dc.format 114 s., 10 s. príloh. cs
dc.format.extent 1448658 bytes cs
dc.format.mimetype application/pdf cs
dc.language.iso cs
dc.publisher Univerzita Tomáše Bati ve Zlíně
dc.rights Bez omezení
dc.subject B-komplex cs
dc.subject doplnky stravy cs
dc.subject chromatografia cs
dc.subject HPLC cs
dc.subject B-complex en
dc.subject dietary supplements en
dc.subject chromatography en
dc.subject HPLC en
dc.title Chromatografické stanovenie B-komplexu cs
dc.title.alternative Chromatographic determination of B-komplex en
dc.type diplomová práce cs
dc.contributor.referee Škrovánková, Soňa
dc.date.accepted 2009-06-02
dc.description.abstract-translated The aim of this study was to test possibilities of determination of B-complex using High Performance Liquid Chromatography and in the case of successful determination to suggest methods and chromatographic conditions, its application on selected dietary supplements. B group vitamins were determined by HPLC method with UV detection at 204 and 220 nm. Separation was performed on column SUPELCOSIL LC 8(15 cm x 4,6 mm; 5 um). Mobile phase was compound of methanol and KH2PO4 (pH 7) in the rate of 10:90. Elution passed in isocratic mode, selected flow rate was 0,8 ml.min-1. Introduced method was applied on six selected dietary supplements. en
dc.description.department Ústav potravinářského inženýrství cs
dc.description.result obhájeno cs
dc.parent.uri http://hdl.handle.net/10563/202 cs
dc.parent.uri http://hdl.handle.net/10563/220 cs
dc.thesis.degree-discipline Technologie, hygiena a ekonomika výroby potravin cs
dc.thesis.degree-discipline Technology, hygiene and economics of food production en
dc.thesis.degree-grantor Univerzita Tomáše Bati ve Zlíně. Fakulta technologická cs
dc.thesis.degree-grantor Tomas Bata University in Zlín. Faculty of Technology en
dc.thesis.degree-name Ing. cs
dc.thesis.degree-program Chemie a technologie potravin cs
dc.thesis.degree-program Chemistry and food technologies en
dc.identifier.stag 12771
dc.date.assigned 2009-02-20
utb.result.grade A


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