| dc.contributor.advisor |
Rouchal, Michal
|
|
| dc.contributor.author |
Fornůsková, Petra
|
|
| dc.date.accessioned |
2013-10-09T11:09:26Z |
|
| dc.date.available |
2013-10-09T11:09:26Z |
|
| dc.date.issued |
2012-02-10 |
|
| dc.identifier |
Elektronický archiv Knihovny UTB |
cs |
| dc.identifier.uri |
http://hdl.handle.net/10563/23121
|
|
| dc.description.abstract |
Hlavním cílem předložené práce bylo optimalizovat podmínky pro rychlé a efektivní stanovení 2,6,9-trisubstituovaných purinů s adamantanovým skeletem metodou LC-MS/MS. Studované sloučeniny vykazují schopnost inhibovat cyklin-dependentní kinázy a také dva typy nádorových buněčných linií. Práce se zabývá syntézou boheminu, od jehož struktury jsou zkoumané sloučeniny odvozeny, a to dvěma různými cestami s cílem srovnat celkový výtěžek obou použitých cest. Dále pak popisuje optimalizaci metody HPLC s UV/Vis detekcí, která předcházela optimalizaci podmínek pro stanovení studovaných sloučenin metodou LC-MS/MS. |
cs |
| dc.format |
69 s. (12294 slov) |
cs |
| dc.format.extent |
3346359 bytes |
cs |
| dc.format.mimetype |
application/pdf |
cs |
| dc.language.iso |
cs |
|
| dc.publisher |
Univerzita Tomáše Bati ve Zlíně |
|
| dc.rights |
Bez omezení |
|
| dc.subject |
purinové inhibitory CDK
|
cs |
| dc.subject |
HPLC- UV/Vis
|
cs |
| dc.subject |
LC-MS/MS
|
cs |
| dc.subject |
adamantan
|
cs |
| dc.subject |
purine inhibitors of CDK
|
en |
| dc.subject |
HPLC-UV/Vis
|
en |
| dc.subject |
LC-MS/MS
|
en |
| dc.subject |
adamantane
|
en |
| dc.title |
Vývoj a optimalizace podmínek pro stanovení purinových sloučenin metodou LC-MS |
cs |
| dc.title.alternative |
Development and Optimization of LC-MS Determination of Substituted Purines |
en |
| dc.type |
diplomová práce |
cs |
| dc.contributor.referee |
Rudolf, Ondřej |
|
| dc.date.accepted |
2012-06-11 |
|
| dc.description.abstract-translated |
The essential goal of the presented diploma thesis was to develop and optimize conditions for the rapid and effective determination of 2,6,9-trisubstituted purines bearing adamantane moiety by LC-MS/MS technique, because there has been demonstrated their ability to inhibit cyclin-dependent kinases and two types of tumor cell lines. This work deals with the synthesis of bohemine by means of two ways and comparing the overall yields of these two pathways. Finally, there is described the optimization of the HPLC with UV/Vis detection, which precedes the optimization of conditions for determination studied compounds using LC-MS/MS. |
en |
| dc.description.department |
Ústav chemie |
cs |
| dc.description.result |
obhájeno |
cs |
| dc.parent.uri |
http://hdl.handle.net/10563/199
|
cs |
| dc.parent.uri |
http://hdl.handle.net/10563/220
|
cs |
| dc.thesis.degree-discipline |
Chemie potravin a bioaktivních látek |
cs |
| dc.thesis.degree-discipline |
Food Chemistry and Chemistry of Bioactive Substances |
en |
| dc.thesis.degree-grantor |
Univerzita Tomáše Bati ve Zlíně. Fakulta technologická |
cs |
| dc.thesis.degree-grantor |
Tomas Bata University in Zlín. Faculty of Technology |
en |
| dc.thesis.degree-name |
Ing. |
cs |
| dc.thesis.degree-program |
Chemie a technologie potravin |
cs |
| dc.thesis.degree-program |
Chemistry and Food Technologies |
en |
| dc.identifier.stag |
27074
|
|
| utb.result.grade |
A |
|
| dc.date.submitted |
2012-05-07 |
|
